LncRNA HAND2-AS1 exerts anti-oncogenic effects on bladder cancer via restoration of RARB as a sponge of microRNA-146

Abstract Background Growing evidence has shown that long noncoding RNA: microRNA: mRNA is implicated in tumor initiation, development, and progression. Long RNA HAND2-AS1 exhibits anti-cancer effects diverse cancers. However, the knowledge of HAND-AS1 bladder cancer development remains unknown. Methods LncRNA miRNA microarray was conducted to explore different expressed primary specimens. RNA-RNA interaction prediction tools miRcode ( http://www.mircode.org/ ), DIANA-lncBase v2 https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=lncbasev2%2Findex-experimental DIANA-TarBase v.8 https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=tarbasev8%2Findex ) miRDB http://www.mirdb.org/ were employed predict interactions between RNA. Bladder cell lines used perform proliferation apoptosis assays. Western blot quantitative Real-time Polymerase Chain Reaction determine expression protein separately. Dual-luciferase assay activity three prime untranslated region retinoic acid receptor beta (RARB). Furthermore, 5637 human mouse models established investigate lncRNA: miRNA: vivo. Results Based on RT2 lncRNA PCR Arrays analysis, we validated declined negatively correlated with depth invasion grades. The overexpression cells RT4 hampered by provoking Caspase 3-triggered apoptosis. Besides, one sponges, miR-146, elevated targeted suppressor, We further demonstrated HAND2-AS1: miR-146: RARB complex promoted 3-mediated suppressing COX-2 expression. Finally, results gained xenografts suggested diminished miR-146 expression, thereby reversing suppression RARB-mediated contributing regression. Conclusion present study sheds light fact exerted as a suppressor releasing from leading inhibition. findings expanded HAND2-AS-mediated regulatory networks' provided novel insights improve RARB-targeted regimens against cancer.